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Phlebotomy Quick Reference Card

Order of draw, tourniquet rules, PPE sequence, and every other number you need for the NHA CPT exam — on one printable page.

Order of Draw (CLSI)

1. Blood culture tubesSterile — always drawn first
2. Light blue (citrate)Coagulation — invert 3-4×
3. Red / Gold (SST)Serum chemistry — invert 5×
4. Green (heparin)Plasma chemistry — invert 8-10×
5. Lavender (EDTA)CBC, blood bank — invert 8-10×
6. Gray (sodium fluoride)Glucose, lactate — invert 8-10×

Tourniquet Rules

Max application time1 minute
If exceededRelease, wait 2 min, reapply
Release pointAs soon as blood flow is established
Risk if left too longHemoconcentration — falsely elevated results

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